Publications on methods:
Light Microscopy
- Spacek J (1989) Dynamics of the Golgi method: a time-lapse study of the early stages of
impregnation in single sections. J. Neurocytol. 18:27-38.
- Spacek J (1992) Dynamics of Golgi impregnation in neurons. Microscopy Research and
Technique 23:264-274.
Ultrastructure - in vivo
- Spacek J (1974) A simple method for the selection of specific small area of central nervous
tissue for electron microscopy. Stain Technol. 49(2):93-6.
Ultrastructure - in slice
- Jensen FE, Harris KM (1989) Preservation of neuronal ultrastructure
in hippocampal slices using rapid microwave-enhanced fixation. J.Neurosci.Methods.
29:217-230. (1,195K
PDF)
- Feinberg, MD, Szumowski KM, Harris KM (2001) Microwave fixation of rat hippocampal slices.
In: RT Giberson, RS DeMaree Jr. (eds.) Microwave Techniques and Protocols. Humana Press: Totowa, New Jersey. pp. 75-88.
(4,079K
PDF)
Serial EM and Reconstruction
- Spacek J, Lieberman AR (1974) Three-dimensional reconstruction in electron microscopy of
the central nervous system. Sbor.ved. praci Hradec Kralove 17:203-222.
- Harris KM, Stevens JK (1988) Study of dendritic spines by
serial electron microscopy and three-dimensional reconstructions. In: Intrinsic
determinants of neuronal form and function. Neurology and Neurobiology
Volume 37 (Lasek RJ, Black MM eds), pp 179-199. New York: Alan R. Liss. (9,320K PDF)
- Carlbom I, Terzopoulos D, Harris KM (1993) Computer-assisted
registration, segmentation, and 3D reconstruction from images of neuronal
tissue sections. IEEE Transaction of Medical Imaging 13(2):351 (7,044
PDF)
- Harris KM (1994) Serial electron microscopy as an alternative
or complement to confocal microscopy for the study of synapses and dendritic
spines in the central nervous system. In: Three-dimensional confocal microscopy:
volume investigation of biological specimens (Stevens JK, Mills LR, Trogadis
JE eds), pp 421-445. New York: Academic Press, Inc. (9,145K
PDF)
- Spacek J (1994) Design CAD-3D: a useful tool for 3-dimensional reconstructions in
biology. J. Neurosci. Methods 53:123-124. (197K
PDF)
- Fiala JC, Harris KM (2001) Extending unbiased stereology of brain ultrastructure
to three-dimensional volumes. Journal of the American Medical Informatics
Association. 8(1):1-16. (1,437K
PDF)
- Fiala JC, Harris KM (2001) Cylindrical diameters method for calibrating
section thickness in serial electron microscopy. J Microscopy 202(3):468-472.
(247K
PDF)
- Fiala JC, Harris KM (2002) Computer-based alignment and reconstruction of
serial sections. Microscopy and Analysis. USA Edition. 52:5-7. (414K
PDF)
- Fiala JC (2002) Three-dimensional structure of synapses in the brain and on the web.
Proceedings of the 2002 International Joint Conference on Neural Networks, May 12-17, Honolulu, Hawaii,
pp. 1-4. (469K PDF)
Last Updated: 12/12/2003
