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This is how we make up our embedding media for serial sectioning.
| Protocol Final Volume |
Chemicals | Volume to Add | Stock Solutions (% or mM) | Final Concentrations (% or mM) |
|---|---|---|---|---|
|
PB Fixtive: 3.5% Para/0.0% Glut
Final Volume: 10 |
Glutaraldehyde | 0 | 70.0% | 0.0% |
| - | Paraformaldehyde | 3.5 | 10.0% | 3.5% |
| - | CaCl2 | 0 | 1000 | 0 |
| - | MgSO4 | 0 | 2000 | 0 |
| - | Phosephate Buffer | 5 | 200 | 100 |
| - | H2O | 1.5 | 0 | 0 |
|
PB Fixtive: 3.5% Para/0.1% Glut
Final Volume: 10 |
Glutaraldehyde | 0.014285714 | 70.0% | 0.1% |
| - | Paraformaldehyde | 3.5 | 10.0% | 3.5% |
| - | CaCl2 | 0 | 1000 | 0 |
| - | MgSO4 | 0 | 2000 | 0 |
| - | Phosephate Buffer | 5 | 200 | 100 |
| - | H2O | 1.485714286 | 0 | 0 |
|
PB Fixtive: 3.5% Para/0.2% Glut
Final Volume: 10 |
Glutaraldehyde | 0.028571429 | 70.0% | 0.2% |
| - | Paraformaldehyde | 3.5 | 10.0% | 3.5% |
| - | CaCl2 | 0 | 1000 | 0 |
| - | MgSO4 | 0 | 2000 | 0 |
| - | Phosephate Buffer | 5 | 200 | 100 |
| - | H2O | 1.471428571 | 0 | 0 |
|
PB Fixtive: 3.25% Para/0.5% Glut
Final Volume: 10 |
Glutaraldehyde | 0.071428571 | 70.0% | 0.5% |
| - | Paraformaldehyde | 3.25 | 10.0% | 3.3% |
| - | CaCl2 | 0 | 1000 | 0 |
| - | MgSO4 | 0 | 2000 | 0 |
| - | Phosephate Buffer | 5 | 200 | 100 |
| - | H2O | 1.678571429 | 0 | 0 |
|
Phend, Rustoni, Weinberg - like Fixtive:
1% Para/2.5% Glut/0.1% Picric Acid Final Volume: 40 |
Glutaraldehyde | 1.428571429 | 70.0% | 2.5% |
| - | Paraformaldehyde | 4 | 10.0% | 1.0% |
| - | Picric Acid | 3.076923077 | 1.3% | 0.1% |
| - | Phosephate Buffer | 20 | 200 | 100 |
| - | H2O | 11.4950549 | 0 | 0 |
|
Picric Acid Fix (0.05% , 0.2% Picric Acid)
- Sonicate if frozen Final Volume: 100 |
Glutaraldehyde | 0.071428571 | 70.0% | 0.05% |
| - | Paraformaldehyde | 40 | 10.0% | 4.0% |
| - | Picric Acid | 15.38461538 | 1.3% | 0.2% |
| - | Phosephate Buffer | 25 | 400 | 100 |
| - | H2O | 19.54395604 | 0 | 0 |
|
Picric Acid Fix (0.1% , 0.2% Picric Acid)
- Sonicate if frozen Final Volume: 100 |
Glutaraldehyde | 0.142857143 | 70.0% | 0.10% |
| - | Paraformaldehyde | 40 | 10.0% | 4.0% |
| - | Picric Acid | 15.38461538 | 1.3% | 0.2% |
| - | Phosephate Buffer | 50 | 200 | 100 |
| - | H2O | -5.52747 | 0 | 0 |
|
Picric Acid Fix (0.5% , 0.2% Picric Acid)
- Sonicate if frozen Final Volume: 20 |
Glutaraldehyde | 0.142857143 | 70.0% | 0.5% |
| - | Paraformaldehyde | 8 | 10.0% | 4.0% |
| - | Picric Acid | 3.076923077 | 1.3% | 0.2% |
| - | Phosephate Buffer | 5 | 400 | 100 |
| - | H2O | 3.78021978 | 0 | 0 |
| Last Updated: 09/18/2007 |