Quantal Nature of Synaptic Transmission

2/26/01


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Table of Contents

Finishing Monday's Lecture Presynaptic Compartment

Cytoskeletal Elements

Docked Synaptic Vesicles

Presynaptic Specializations

Presynaptic Specializations

Presynaptic Specializations NMJ

Presynaptic Compartment

Quantal Nature of Synaptic Transmission

Question

Overview this lecture

Sherrington - A physiologist's Contribution to Neuron Doctrine

Soup vs Spark

Soup vs Spark Controversy about Synaptic Transmission

Soup vs Spark Controversy

Soup vs Spark Controversy Is synaptic transmission generally chemical or electrical?

Synaptic transmission

NMJ recording electrode

1951 "Quantal" nature of synaptic transmission discovered

Spontaneous miniature endplate potentials (MEPP) - NMJ

Distinct presynaptic vesicles discovered in 1954 by de Robertis and Bennett

Quick Frozen NMJ

Standard Katz (Quantal) Model of Synaptic Transmission

Key Variables

Determining P and Q at NMJ

P and Q at NMJ

Determining N is more difficult

Independence of Q at NMJ

Quantal Properties of some Model Synapses

Synaptic Transmission at CNS synapse

Synaptic Transmission at CNS synapses

Nomenclature of Spontaneous "mini's" at CNS Synapses

Experimental Conditions that affect properties of synaptic transmission

Releasing NX at CNS synapses

Detecting NX released at CNS synapses

Why is knowing N important?

Methods developed in an effort to Study Single CNS Synapses

Spontaneous MEPSC at thalamic neuron (guinea pig brain slice)

Evoked EPSCs reveal quantal transmission in same thalamic neuron

CNS Synapses much more variable Q - quantal size

Variation in Vesicle size is independent of synapse size

Readily Releasable Pool

Docked vs non-docked CNS vesicles

Morphological Correlates of Katz's Quantal Analysis Variables

Friday Discussion Section

Author: Kristen Harris