Table of Contents
Finishing Monday's Lecture
Presynaptic Compartment
Cytoskeletal Elements
Docked Synaptic Vesicles
Presynaptic Specializations
Presynaptic Specializations
Presynaptic Specializations NMJ
Presynaptic Compartment
Quantal Nature of
Synaptic Transmission
Question
Overview this lecture
Sherrington - A physiologist's Contribution to Neuron Doctrine
Soup vs Spark
Soup vs Spark Controversy about Synaptic Transmission
Soup vs Spark Controversy
Soup vs Spark Controversy
Is synaptic transmission generally chemical or electrical?
Synaptic transmission
NMJ recording
electrode
1951 "Quantal" nature of synaptic transmission discovered
Spontaneous miniature endplate potentials (MEPP) - NMJ
Distinct presynaptic vesicles discovered in 1954 by de Robertis and Bennett
Quick Frozen NMJ
Standard Katz (Quantal) Model of Synaptic Transmission
Key Variables
Determining P and Q at NMJ
P and Q at NMJ
Determining N is more difficult
Independence of Q at NMJ
Quantal Properties of some Model Synapses
Synaptic Transmission at CNS synapse
Synaptic Transmission
at CNS synapses
Nomenclature of Spontaneous "mini's" at CNS Synapses
Experimental Conditions that affect properties of synaptic transmission
Releasing NX at CNS synapses
Detecting NX released at CNS synapses
Why is knowing N important?
Methods developed in an effort to Study Single CNS Synapses
Spontaneous MEPSC at thalamic neuron (guinea pig brain slice)
Evoked EPSCs reveal quantal transmission in same thalamic neuron
CNS Synapses much more variable Q - quantal size
Variation in Vesicle size is independent of synapse size
Readily Releasable Pool
Docked vs non-docked CNS vesicles
Morphological Correlates of Katz's Quantal Analysis Variables
Friday Discussion Section
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