Gap Junctions, Electrotonic Synapses

2/28/01


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Table of Contents

Gap Junctions, Electrotonic Synapses

Electrotonic Synapses Discovered

Properties of Gap Junctions (GJ) and Electrotonic Synapses (ES)

Ultrastructure

Molecular Gap Junction Channel Formation

Gap Junction Channels

Freeze-fracture Gap Junction Plaque

Not all Gap Junctions form "Plaques"

Domain Structure of Connexins (> 12 cloned so far)

Major function of Gap Junctions is to regulate brain volume

Gap junction communicating compartments

Distinctions between Chemical and Electrotonic Synaptic Transmission

Bidirectional Electrotonic Junctions

Gap Junction Coupling

Junctional Conductance also determined by amount of transjunctional voltage

Modulated by other factors too

Rectifying Electrotonic Synapse (direction of current flow depends on depolarization status)

No Synaptic Delay - SPEED!

Electrotonic and Synaptic Junctions can be mixed in all combinations on the same cell

Dye-coupling - LM indicator of Gap Junctions

Thalamic - Neocortical Slice Setup

Firing patterns distinguish different types of Cortical interneurons

Chemical Synapses

Review - Begin Here on WEDNESDAY Other studies have shown that cortical Interneurons have

Electrical (Electrotonic) Synapses

Octanol blocks gap junctions and electrical coupling

Action Potentials evoked Spikelets

Frequency stimulation to Cell 1

Electrical Synapses Couple Spiking activity of two interneurons

Electrical Synapses Couple Spikeing activity of two interneurons

Occurrence of Electrical Synapses between Pairs of Neocortical Interneurons

Summary Electrical Synapses

Dermietzel, 1993, Fig. 3A

Dermietzel, 1993, Fig. 4

Author: Kristen Harris