Table of Contents
Synapse Specificity Through Molecular Compartmentalization
~50% of all Ca1 Spines have SER
Cerebellar Spine and SER compartments
Synapse Specificity through Local Protein Synthesis
Polyribosomes in spines
If time also see website
Endocytosis in Spines
Actin not MT's = spine cytoskeleton
Spine Summary
Molecular Composition of the PSD
Postsynaptic Density
Molecular Organization
Illustration of the types of synaptic structures which can be purified from brain
Molecules identified by biochemical purification of the PSD
Glutamate Receptors and their associated proteins
Glutamate receptors and their associated proteins drawn to molecular scale fit easily in the PSD
Relative locations of Glutamate Receptors in the PSD based on Immunogold EM
NMDA receptor
NMDA receptor model
NMDA receptor - PSD-95 Complex
PSD-95
Function NR2 - PSD-95
Function NR2 - PSD-95
NR2 - PSD-95 - nNos
NR2 - PSD-95 - SynGAP
NR2 - PSD-95 - Fyn
NR2 - PSD-95 - GKAP to Shank
NR2 - PSD-95 - MAP1A and Cript
NR1 - gates the Ca channel through its interactions with cytoplasmic proteins
NR1 - actinin - F-actin
NR1 - CaM
NR1 - Yotaio - PP1, PKA
NR1 - F-actin Spectrin
AMPA receptor distribution in the PSD based on Immunogold EM
1 subunit of GluR3 "AMPA" glutamate receptor
Synaptic targeting of AMPA receptors
AMPA - GRIP/ABP
AMPA - GRIP/ABP
AMPA - PICK1
AMPA - NSF-SNAP
Metabotropic Glutamate Receptors in the PSD based on Immunogold EM
mGluR receptor structure
mGlu Receptor - Homer - IP3 receptor
mGluR1 - Homer - IP3
Structural "linkage" of SER at PSD edges
Structural "linkage" of SER at PSD edges
mGluR1 - Shank - NMDA complex
NMDA - Shank - Homer
Excitatory PSD Signaling Microdomains (SM)
Molecular Heterogeneity reflects Structural Diversity of Glutamatergic PSDs
Symmetric (inhibitory) and Asymmetric (excitatory) Synapses (Hippocampus)
Anchoring NX receptors at Inhibitory Synapses
Summary
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