Kristen M. Harris
The University of Texas at Austin
1 University Station, C7000
Austin, TX 78712-0805
Office: NMS 5.306A
Place Of Birth:
Fargo, North Dakota USA
Married, Max Snodderly; Son, Collin
- 1976 B.S. Moorhead State University (now Minnesota State University Moorhead, Summa cum Laude, Biology Major, Chemistry & Math Minors)
- 1979 M.S. in Neurobiology University of Illinois
(Thesis Advisor, William T. Greenough, Ph.D.)
- 1982 Ph.D. in Neurobiology, Northeastern Ohio Universities College of Medicine and Kent State University
(Thesis Advisor, Timothy Teyler, Ph.D.)
- 1982-1984 Department of Neurology, Neurocytology Laboratory, Massachusetts General Hospital, Boston, MA (Sponsor, Dennis Landis, M.D.; Collaborator, John Stevens, University
- 1984 Instructor, Department of Neuropathology, Harvard Medical School,
and Neuroscience Division, Children's Hospital, Boston, MA
- 1985 Assistant Professor, Dept. of Neurology at Children’s Hospital, and the Program in Neuroscience, Harvard Medical School.
- 1996-1999 Associate Professor, Dept. of Neurology at Children's Hospital and the
Program in Neuroscience at Harvard Medical School, Boston, MA
- 1999-02 Professor, Biology Department, Boston University, Boston, MA
- 2000-02 Co-Director, Program in Neuroscience, Boston University, Boston, MA
- 2002-04 Adjunct Professor, Biology Department, Boston University, Boston, MA
- 2002-06 Professor, Neurology, Medical College of Georgia, Augusta, GA
- 2002-06 Georgia Research Alliance Eminent Scholar
- 2002-04 Chief, Synapses and Cell Signaling Program, Institute of Molecular Medicine and Genetics, Medical College of Georgia, Augusta, GA
- 2004-06 Director, Synapses and Cognitive Neuroscience Center, Medical College of Georgia, Augusta, GA
- 2006- Adjunct Professor, Department of Neurology, Medical College of Georgia, Augusta, GA
- 2006- Professor, Section of Neurobiology, Fellow in the Center for Learning and Memory, University of Texas, Austin TX
Awards and Honors:
- 1980 NIH Individual Predoctoral Fellowship
- 1980 Scholarship, Neurobiology Course, Woods Hole, MA
- 1980 Sigma Xi Grant in Aid of Research
- 1982 NIH Individual Postdoctoral Fellowship
- 1987 Milton Fund Grant, Harvard Medical School
- 1987 Outstanding Young Alumna Award, Minnesota State University Moorhead
- 1987 Sloan Research Fellowship, Alfred P. Sloan Foundation
- 1992 Finalist, Computerworld Smithsonian Award, Image Graphics Laboratory
- 1993-94 Judge, Computerworld Smithsonian Awards
- 2002 Packard Foundation Grant
- 2002-04 Councilor, Society for Neuroscience
- 2003 Distinguished Alumna Award, Minnesota State University Moorhead
- 2005- Javits Merit Award, NINDS
Memberships and Committee Assignments:
University of Texas at Austin
- National Institutes of Health and National Institutes of Mental health:
- 1990-95 Consultant for Neurology Study Section B - Program Project Grants
- 1992-95 Consultant National Resource Center Grants
- 1996-97 Member, Cognitive Functional Neuroscience Review Committee, NIMH
- 1997-99 Member, Molecular and Cellular Developmental Neurosciences 1 Study
- 2000- Ad Hoc Reviewer for several Study Sections
- National Science Foundation:
- Professional Societies:
- 1976- Society for Neuroscience (SFN)
- 1993 Organizer, Social on Neuroanatomical Methods
- 1998 Chair Symposium on Dendritic Spines
- 2000-04 Councilor
- 2000 Member Search Committee for new Executive Director of SFN
- 2002-04 Co-chair Professional Development Working Group
- 2004- Founding member, Neuroinformatics Committee
- 1976-2004 Women in Neuroscience (WIN)
- 2001 Chair, Mika Salpeter WIN Lifetime Achievement Award committee
- 2004 Fostered WIN merger into the SFN
- 1978 Sigma Xi
- 1980 Association for Women in Science
- 1985 New England Society for Electron Microscopy
- 1991- American Association of Anatomists
- 1991- Microscopy Society of America
- 1993- American Association for University Women
- 1998 Organizing Chair, Keystone Symposium on Synapse Formation and Function
- 2000 Organizing Chair, Gordon Research Conference on Cell Biology of Neuron
- 2002- Steering Committee, Gordon Research Conference on Neural Plasticity
Medical College of Georgia:
- 2007- Chair, Graduate Student Admissions Committee – Institute for Neuroscience
- 2006- Member, Search Committee – Center for Learning and Memory
Member, Curriculum committee – Institute for Neuroscience
Assistant Graduate Student Advisor – Institute for Neuroscience
Member, Executive Committee – Institute for Neuroscience
- 2004-06 Member, Steering Committee, Residency Program in Psychiatry
- 2004-06 Member, Steering Committee, Institute of Neuroscience
- 2002-06 Member, Executive Committee, Program in Neuroscience PhD
- 2002-04 Chair, Search Committee, Synapses and Cognitive Neuroscience Center
- 2002-04 Member, Leadership Council, MCG Neuroscience Center of Excellence
- 2002-04 Member, Neuroscience Eminent Scholar Search Committee
- 2002-04 Judge, Postdoctoral Fellow Research Day Seminars
- 2002-04 Chair, Program in Neuroscience PhD Development Committee
- 2004-06 Member, Executive Committee, PhD Program in Neuroscience
- 2004-06 Member, Steering Committee, Institute for Neuroscience
Harvard Medical School:
- 1999-02 Member, Neuroscience Faculty Search Committee
- 2000-02 Chair, Neuroscience Seminar Series
- 2000-02 Member and then Chair, Biology Library Committee
- 2000-01 Co-Chair, Neuroscience and Cell Molecular Search Committee
- 2000-02 Member, Search Committee for Dean of the College of Arts and Sciences
- 2000-02 Member, Academic Promotions and Tenure Committee, Biology
- 2000-02 Member, Steering Committee, Biomedical Engineering Training Grant
- 2000-02 Member, Steering Committee, Whitaker Foundation Grant, Biomedical Engineering Department
- 2002 Member, "Faculty Roundtable" for Boston University Viewbook
- 1987-90, 93 Admissions Committee, Program in Neuroscience
- 1989-92 Neurology Training Grant Executive Committee
- 1990-99 Leadership role in establishing the Department of Research Computing,Member Steering committee
- 1992, 94, 97 Search Committee Member for Director of Research Computing
- 1994-95 Enders Faculty Council, Alternate member for Neuroscience
- 1994 Neuroscience Seminar Series Coordinator
- 1991-99 Director, Image Graphics Core Laboratory, Children's Hospital
- 1994-99 Director, Electron Microscopy Core Laboratory, Department of Neurology/Neuroscience.
- 2002- Journal of Neuroscience Methods
- 2002- Hippocampus
Brain Cell Biology, Formerly Neurocytology
- 2002- Neuroinformatics
- 1987- Journal of Comparative Neurology
- 1989- Brain Research
- 1990- Journal of Neuroscience
- 1992- Hippocampus
- 1993- Science
- 1993- Journal of Electron Microscopy Techniques
- 1994- Proceedings National Academy of Sciences
- 1995 Nature
- 1998- Nature Neuroscience
- 1999- Neuron
- 2004- PLOS
- 2004- Cerebral Cortex
- 2004- Histology and histopathology
- 2004- European Journal of Neuroscience
Research Interests and Accomplishments:
Our goal is to elucidate structural components involved in the cell biology of learning and memory. We study long-term potentiation (LTP) and its complement long-term depression (LTD) in the developing and mature hippocampus because these phenomena have many of the physiological characteristics that are expected for learning and memory in the brain. Our working hypothesis is synaptic plasticity that serves to modify synapses in the creation of new memories competes with homeostatic mechanisms that serve to prevent saturation of synaptic strength and neuropathology. Our focus has been on dendritic spines because they are the major postsynaptic targets of excitatory axons throughout the brain and because their structure and composition serve both synaptic plasticity and stabilizing homeostatic mechanisms.
Dendritic spines and their synaptic components are too small to measure accurately with light microscopy so we have developed and standardized computer-assisted approaches to analyze them in three-dimensions through serial section transmission electron microscopy (ssTEM). We have established the rat hippocampal slice as a model system for structural synaptic plasticity by developing a microwave-enhanced procedure that produces rapid fixation to the center of the slice within a minute after the last physiological recording. We have collaborated with Dr. Sergei Kirov to use two-photon and confocal microscopy to visualize global effects of different levels of synaptic activity on spine density along dendrites prior to ultrastructural analyses. We have received a Javits Merit award to extend our studies regarding the functional ultrastructure of LTP into the hippocampus of the awake rat in collaboration with Dr. Wickcliffe Abraham at the University of Otago, Dunedin, New Zealand. We collaborate with Dr. Michael Ehlers at Duke University to understand the dynamics and identity of organelles, such as recycling endosomes and Golgi apparatus in dendrites and at synapses.
Our research has revealed contrasting effects of synapse activation on spine structure and formation in the immature and mature rat hippocampus. Prior to postnatal day (PN) 11, hippocampal CA1 synapses are located on the dendritic shafts or along dendritic filopodia, often piled up around the base of a filopodium. By PN15, shorter dendritic spines have emerged with enlarged heads each of which hosts one synapse, filopodia are rare, and shaft synapses have diminished almost to their low (<5%) adult level. In parallel, we have shown that LTP develops during PN11 to 15 after dendritic spines have begun to emerge. Recent studies from our lab show that if LTP is induced at PN15, the predominant location of polyribosomes shifts from dendritic shafts into dendritic spines and those spines containing polyribosomes have enlarged synapses by 2 hours after induction. Our recent findings show a robust increase in the number of dendritic spines with synapses, nonsynaptic filopodia, and polyribosomes throughout the dendrites by 5 and 30 minutes after induction of LTP. Spine number returns to control levels by 2 hours suggesting a rapid competition and elimination for synaptic sites during 1-2 hours after induction of LTP. These findings provide strong evidence for local changes in protein synthesis at a subpopulation of synapses on developing hippocampal neurons during LTP. Like PN15, mature hippocampal dendrites also show a synapse specific increase in size at 2 hours after the induction of LTP associated with a local elevation in polyribosomes. With Dr. Abraham we will determine how soon, and for how long after induction of LTP the synapses remain altered in vivo in the mature hippocampus to provide a stable mechanism of enduring LTP.
We have measured dendritic spines and their presynaptic partners in several mature brain regions. We established that interneurons do not synapse with hippocampal dendritic spines, thereby removing differences in presynaptic input types as a potential source of the large (>10 fold) variation in spine dimensions along even a short 5-micron long segment of dendrite. Our findings show that larger spines have proportionately larger and more complex subcellular organelles, and postsynaptic receptive surfaces, and more presynaptic vesicles. We have shown that spine necks are constricted just enough to allow the heads to be relatively isolated biochemical compartments near the synapses, without choking off transmission of electrotonic signals to the postsynaptic dendrite. Furthermore, our recent studies show that different subsets of spines contain smooth endoplasmic reticulum, or endosomal compartments suggesting the independence of these organelles in modulating synaptic efficacy. These findings demonstrate the power of ssTEM to capture and illustrate dynamic processes on the ultrastructural scale by comparing across ages, conditions, times, dendrites, and brain regions.
Studies using confocal and two-photon microscopy in collaboration with Dr. Kirov show that blocking synaptic transmission on mature hippocampal neurons results in a prodigious up regulation in the number of dendritic spines. These findings suggest that a homeostatic mechanism was triggered to maintain a constant level of synaptic input on mature neurons. In contrast, if synaptic transmission was blocked at ages younger than postnatal day 21, there was no net effect on spine number. It appears that the manifestation of this homeostatic mechanism requires a substantial period of postnatal development. Three-dimensional studies at the ultrastructural level demonstrated a recapitulation of development in the formation of new synapses during conditions of blocked synaptic transmission in the adult.
Others have shown that astrocytic processes are heavily endowed with glutamate transporters and neurons grown in culture without astrocytes are 12 fold less active than neurons grown with glia. Therefore, measuring the degree to which astroglia surround and intervene between synapses is integral to understanding their role in synaptic transmission and plasticity. We found that about 50% of hippocampal synapses have astrocytic processes surrounding a portion of their perimeters and those synapses that have perisynaptic astroglia are substantially larger than those without. LTP induces filopodia which eventually become dendritic spines, but the new smaller spines have no perisynaptic astroglia.
Areas in which I plan to expand our research include models of learning and memory and mental retardation in vivo, the role of sleep and circadian rhythms in modulating synapse number and structure, and mapping the location of key molecules in three dimensions. In addition, I am working to understand the role of protein synthesis in structural synaptic plasticity. In earlier work, I showed that LTP has a circadian cycle, being more likely to occur at a higher magnitude during the rat’s sleep cycle for hippocampal area CA1 and during the rat’s active wake cycle in hippocampal area dentata. One goal will be to determine whether circadian and sleep rhythms in the mature nervous system influence spine formation and/or preservation after LTP, LTD, or learning and memory. I also plan new efforts towards producing accurate three-dimensional maps of relevant molecules at the synapse, along dendrites, presynaptically, and in relationship to astroglial processes. In addition, I plan to expand our database of dendrites and synapses (see synapses.clm.utexas.edu); and to implement automatic alignment and segmentation algorithms in collaboration with Dr. Dmitri Chklovski (Janilia Farms) and Chadrajit Bajaj (UT-Austin).
Current Grant Support:
- 07/01/84 – 06/30/12 PI, Spine & Synaptic Plasticity in Mature Hippocampus.
RO1 NS21184 Current year direct costs $218,727
Received 7 year Javits Merit award, beginning 07/01/05.
- 05/01/96 - 05/31/08 PI, Ontogeny of Structure and Function at Hippocampal Synapses.
RO1 NS33574; Current year direct costs: $213,750
- 09/30/97 - 04/30/08 PI, 3-Dimensional Structure and Function of Synapses in the Brain.
RO1 EB002170, formerly grant number MH/DA57351; under the auspices of the Human Brain Project. Current year direct costs: $175,000.
Past Grant Support:
- 09/01/99 - 08/31/04 Co-PIs, K. Harris, M. Teich and B. Selah
Functional Imaging of Synapses by Entangled-Photon Microscopy.
Packard Foundation: 5-year period direct costs: $960,000
- a) University Courses
- 1976-77 Premedical Biology, University of Illinois
- 1980 Functional Neuroanatomy, Northeastern Ohio Universities College of Medicine
- 1982 Workshop on Hippocampal Slices, Fred Haer Institute, Maine.
- 1987-88 Information Processing and Behavior, Harvard Medical School (HMS)
- 1988 Advanced Neurobiology, Program in Neuroscience, HMS.
- 1989-90 Introduction to Neuroscience Research, Program in Neuroscience, HMS
- 1990 Conduct of Science, Graduate Studies, Division of Medical Sciences, HMS
- 1992 Advanced Topics in Neurobiology, Structure of Synaptic Function", HMS
- 2001 Neurobiology of the Synapse, Biology, BU
- 1999-2004 Selected lectures in Fundamental Neuroscience courses, BU, MCG
- 2008 Neurobiology of Synapses, UT-Austin
- b) Postdoctoral Fellows and Research or Visiting Professors:
- 2004- Postdoctoral Research Fellow, Jennifer Bourne, PhD performs experiments on synaptic plasticity in mature brain slices and in vivo.
- 2003- Research Assistant Professor, Bitao Shi, MD, is currently a member of our research team with extensive training both in neurology and database creation and management. He is developing our Neuroinformatics Database on Synapses and Dendritic Spines.
- 1993- Fogarty Fellow and Visiting Professor, and Current Collaborator, Dr. Josef Spacek, Professor, Dept. Pathology, Hradec Krolove, Czech Rep. - made 3 extended visits, resulting in three major publications regarding the ultrastructural composition of dendritic spines and their synapses. We continue our collaboration on original research as well as our Website Tutorials, Ultrastructural Neuropathology, and Neuroinformatics Synapses database.
- 2000-01 Visiting Postdoctoral Fellow, Matthew Xu-Friedman, PhD from Dr. Wade Regehr’s laboratory at Harvard Medical School. Co-authored a paper.
- 1999-2000 Postdoctoral/Neurology Fellow, Carlos Portera-Cailliau, MD-PhD investigated the role of dendritic filopodia in synapse formation in CA1. Currently, moving to a new position as Assistant Professor-in-Residence at the David Geffen School of Medicine At UCLA.
- 1997-99 Postdoctoral Research Associate, Dr. John Fiala developed our reconstruction software (entitled RECONSTRUCT”) in my lab. This software is freely available at synapses.bu.edu or synapses.clm.utexas.edu. When I moved to Boston University, Dr. Fiala became a Research Assistant Professor affiliated with my laboratory there. He remains as such in the Department of Biology at Boston University and we continue to collaborate on projects.
- 1997-99 Postdoctoral Research Associate, Sergei Kirov, PhD is an expert physiologist and multiphoton imager who has collaborated with me on numerous studies. Currently, Assistant Professor, Neurosurgery, Medical College of Georgia. Recipient of an NIH – KO1 award.
- 1997-98 Postdoctoral/Neurology Fellow, Gordon Shepherd, Jr., MD/PhD. Co-authored elegant paper demonstrating the variation and composition of presynaptic axons through 3-dimensional reconstructions. Currently Postdoctoral Fellow with Dr. Karel Svoboda, will be moving as an Assistant Professor to Northwestern University in the Department of Physiology.
- 1997-99 Postdoctoral Fellow, David Selig, MD, NRSA fellow investigating the structural basis of synaptic transmission. Currently in anesthesiology residency.
- 1996-97 Visiting Professor, Dr. Viktor Popov, Senior Scientist at Institute of Cell biophysics, Russian Academy of Sciences in Pushchino, Russia.
- 1991 Postdoctoral fellow, Dr. Patricia Suppes, co-authored major paper before assuming full-time clinical and research duties at the University of Texas in Dallas.
- 1986-87 Postdoctoral Fellow, Dr. Frances Jensen; co-authored several papers, obtained a Physician Scientist Award, and NRSA funding based on application written in my laboratory. Currently Associate Professor in the Div. of Neuroscience, Dept. of Neurology, Children's Hospital.
- c) Predoctoral Graduate students:
- 2006- Visiting Students or Research Staff
- - Jared Bowden, PhD candidate, Univ. Otago, Dunedin NZ in the laboratory of Dr. W. C. Abraham.
- - Davi Bock, PhD candidate, Harvard Medical School, in the laboratory of Dr. Clay Reid.
- - Cam Robinson, Staff Scientist, Duke University Medical Center, in the laboratory of Dr. Michael Ehlers
- 2006- Thesis advisor or Rotation supervisor, Graduate Students at UT- Austin
- - Priyanka Godbole, MS student in collaboration with Dr. Chandrajit Bajaj
- - Masson Weems, PhD student in collaboration with Dr. Chandrajit Bajaj
- 2003-06 Thesis advisor or Rotation supervisor, Graduate Students at the Medical College of Georgia (MCG)
- Mark Witcher (Thesis advisor, MD/PhD student working on the role of perisynaptic astroglia in synaptogenesis in the mature rodent brain; and in human brain slices, to study medial temporal lobe epilepsy)
- William Risher (PhD Neuroscience student worked on the role of perisynaptic astroglia in synaptogenesis during development and LTP)
- Jennifer Salgado (PhD Neuroscience student worked on role of recycling endosomes in rapid spine enlargement and spine formation during LTP)
- Jackie Tilsner (PhD Neuroscience student)
- Seungshin Ma (PhD Neuroscience rotation, Thesis committee member)
- Vanessa Bundy (PhD rotation student, 2003)
- 2000- Thesis advisor or Rotation supervisor, Graduate Students at BU
- Linnaea Ostroff (Thesis advisor, Ph.D. Prog. In Neuroscience BU, moved to MCG to complete her thesis, defended, November, 2004)
- Linda Caparell (Masters Thesis advisor, Biology/Neuroscience at BU)
- Tom Giove (Rotation supervisor, MCBB Program at BU)
- Mark Seid (Grad. Student, Co-Advisor with Dr. James Traniello, BU), Currently Postdoctoral Fellow with Rüdiger Wehner in Switzerland
- 1988-99 Thesis advisor (T) or Rotation (R) supervisor, Graduate Students at HMS
- Karin Sorra (T, Ph.D. Prog. In Neuroscience, Recipient Canadian foreign fellowship, several first author papers)
- Marina Chicurel (R, Ph.D. Prog. in Neuroscience, Hughes Fellowship awarded while in my lab, first author paper)
- Paul Jackson (R, M.D. Ph.D. Prog. in Neuroscience, first author paper)
- Alo Basu (R, Ph.D., Prog. in Neuroscience)
- Alex Carter (R, MD/Ph.D., Prog. in Neuroscience)
- Bruce Peters (R, Ph.D. Prog. in Neuroscience)
- Mary Morris (R, Ph.D. Prog. in Neuroscience)
- Michael Levin (R, Ph.D., Cell and Dev. Biol.)
- Gerri Martin (R, Ph.D. Prog. in Neuroscience)
- 1998 Visiting graduate student
- David MacMahon, from University of Pittsburgh, co-authored a paper.
- 1993- Outside examiner for Ph.D. Theses:
- C. Woolley (B. McEwen, Advisor, Rockefeller Univ., New York, 1993).
- Member of an International Committee to examine 6 Ph.D. candidates in the Laboratory of Prof. Per Andersen, Univ. Oslo, Norway (1995)
- K. Leslie (G. Turringiano, Advisor, Brandeis Univ., Boston, MA, 2000)
- d) Undergraduate Honors Students and Research Assistants en route to professional school:
- 2002-2003 Medical College of Georgia
- Anusha Mishra, Research Assistant, Applying for MD/PhD programs
- Jeremy Smalley, Summer MCG STAR student
- Jamie Hurlburt, Research Project Manager, currently in DO medical school program in Maine.
- 2000-02 Boston University
- Priya George, Biology honors student
- James Cooney, Biology, Undergraduate Research Opportunity research grant (UROP), Work for Distinction Honors Thesis on endosomes in dendrites, First author, J. Neurosci. 2002. Enrolled in Medical School.
- Victoria Hui, Biomedical Engineering, had a UROP in my lab
- Lara Petrak, now at HMS imaging core.
- Alex Goddard, now HMS PhD student, Neuroscience
- 1987-99 Harvard Unversity
- Rachel Ventura (Currently at Columbia, Co-authored paper)
- Beatrice Tsao (Radcliffe Project Grant, Medical School, NYU, co-authored 2 papers, now a practicing physician)
- Peter Sultan (Medical School, Cornell, NY, co-authored paper)
- Katrien Neukermans (Medical school, Johns Hopkins)
- Greg Belmont (Graduate school in Neuroscience, MIT) Andrew Nguyen (MD/Ph.D. Prog., Univ. Calif., San Diego).
- 1996-98 Judge and Mentor, Center for Excellence in Education
Regional, National and International Contributions
- 1985-97 Gave 43 invited seminars.
- Organizer, Boston Area Hippocampus Club January, 1998 Meeting
- Organizer, Keystone Symposium on Synapse Formation and Function.
- Discussant, NIH workshop on Brain Molecular Anatomy Project
- Speaker, SUNY Health Science Center
- Speaker, Spring Meeting on the Human Brain Project
- Speaker, Gordon Conference on the Cell Biology of the Neuron
- Lecturer, Cold Spring Harbor.: Imaging Structure and Function in the Nervous system
- Lecturer, Woods Hole Neurobiology Course
- Symposium Organizer and Speaker: Dendritic Spines, Society for Neuroscience
- Lecturer, Harvard Medical School Course in Neuroanatomy
- Speaker, Northeastern Ohio College of Medicine
- Speaker, Mount Sinai Medical School
- Speaker, Baylor College of Medicine
- Speaker, Boston University Neuroscience Day
- Speaker, Biology Department Retreat
- Chair of session, Gordon Conference on Neural Plasticity
- Speaker, Massachusetts Institute of Technology, Brain and Cognitive Neuroscience
- Speaker, Harvard Medical School, Neurobiology Seminar Series
- Speaker, NYU - Neuroscience Seminar
- Organizer and Speaker, Boston Area Hippocampus Club
- Chair, Gordon Conference on the Cell Biology of the Neuron
- Lecturer, Marine Biological Laboratory Summer Neurobiology Course
- Speaker, Cold Spring Harbor, Neuroscience Seminars
- Speaker, Medical College of Georgia
- Speaker, Gordon Research Conference on Excitatory Amino Acids - Italy
- Speaker, Conference on Databasing in the Brain, Norway
- Speaker, Gordon Research Conference on Neuroplasticity, Rhode Island
- Lecturer, Marine Biological Laboratory Summer Neurobiology Course
- Speaker, Brown University, Neuroscience, Dept., Rhode Is.
- Speaker, Cognitive and Neural Systems Dept., Boston University
- Keynote Speaker, Wash. Univ. Neuroscience Retreat, St. Louis
- Speaker, Anatomy and Neurobiology Department, Boston University
- Speaker, Winter Conference on Brain Research, Snow Mass, Colorado
- Helen Molinari Memorial Lecturer, Albany, NY
- Speaker, Neurobiology and Behavior, University of California, Irvine, CA
- Speaker, Columbia Neuroscience, NY
- Speaker, Medical College of Georgia
- Speaker, University of Pittsburgh
- Speaker, Gordon Research Conference on Synaptic Transmission, New Hampshire
- Speaker, NIDA Satellite Symposium, Frontiers in Addiction Research
- Speaker, Human Brain Project Symposium, Society for Neuroscience
- Speaker, AAAS meeting in Denver, CO.
- Speaker, Department of Neurobiology, Yale University
- Weirsma Visiting Professor, Cal Tech, CA
- Speaker, MIT Center for Learning and Memory, MA
- Speaker, 30th Anniversary. Discovery of LTP, Meeting of the Royal Society in London
- Speaker, Satellite Symposium on Synaptogenesis, Austrian Neuroscience Association, Vienna
- Speaker, Symposium on the Molecular Dynamics of Synapse Assembly and Plasticity, Inter’l Brain Research Org. Meeting, Prague Czech Republic
- Speaker, University of Maryland, Baltimore
- Speaker, Rutgers University, New Jersey
- Speaker, Human Brain Project, Satellite Symposium, Society for Neuroscience Meetings, New Orleans
- Speaker, Emory University, Atlanta
- Speaker, Synapse: Molecular Mechanisms of Plasticity, Symposium to celebrate opening of the Porter Neuroscience Research Center at the NIH
- Speaker, Gordon Research Conference: Cell Biology of the Neuron, New Hampshire
- Speaker, University of Maryland Baltimore, Baltimore, MD
- Speaker, 14th Neuropharmacology conference on "The Cytoskeleton & Neuronal Plasticity" San Diego, CA
- Speaker, UCLA Joint Seminar in Neurosciences Series, University of California, Los Angeles
- Speaker, Emory University, Yerkes Primate Center, Atlanta, GA
- Speaker, “Ultrastructure of dendritic spines and long-term potentiation”, University of Texas, Austin, TX
- Speaker, University of Washington, Seattle, WA (I)
- Speaker, Cold Spring Harbor Meeting on Learning and Memory, CSHL, New York
- Speaker, Commencement, Minnesota State University, Moorhead Minnesota
- Lecturer, Neurobiology Summer Course Lectures. Woods Hole, MA
- Speaker, Gordon Research Conference, Excitatory Amino Acids, Aussois, France
- Speaker, Winter Conference on Learning and Memory, Park City, Utah
- Speaker, Neuroscience Seminar, Stanford University, CA
- Speaker, Neuroscience 2006 Symposium, Neuroscience Dept. Farmington CT
- Speaker, Cold Spring Harbor Labs - Meeting on Channels, Receptors, and Synapses
- Speaker, Neuroscience Seminar Series, UBC, Vancouver
- Speaker, Brain Research Centre Research Day UBC, Vancouver
- Speaker, Neuroscience Institute, University of Michigan, Ann Arbor
- Lecturer, MBL Neurobiology Course, Woods Hole, MA
- Invited Speaker, Institute of Neuroscience Seminar Series, UT-Austin, TX
- Invited Speaker, 16th Neuropharmacology conference “LTP: Forty Unforgettable Years” Satellite meeting at the Society for Neuroscience, Atlanta.
- Symposium Chair and Speaker, "How Synapse Structure Teaches Us About Function, and Vice Versa." Society for Neuroscience.
- Invited Participant in an event at the SfN headquarters co-sponsored by the Spanish Embassy to commemorate the Centennial of the 1906 Nobel Prize shared by Cajal and Golgi.
- Symposium Chair, Structural Plasticity underlying Learning and Memory Mechanisms. Winter Conference on Learning and Memory, Park city, Utah
- Invited Speaker, Structural Plasticity of Dendrites, Gordon Research Conference on Dendrites: Molecules, Structure, and Function, Ventura Beach, CA
- Apr Speaker, Brown University, Providence, RI
- Apr Speaker, Yale University, New Haven, CT
- May Invited Speaker, "Neuronal morphological plasticity: Mechanism and Meaning, "Fondation des Treilles in Tourtour, France
- June Symposium Speaker and Author in the anticipated book: Structural and Functional Organization of the Synapse, Iowa City, IA
- Sept. Co-organizer, conference on “High-Throughput Neuroanatomy”, Howard Hughes Medical Institute, Janelia Farm Research Campus, MD
- Original Reports in Peer-Reviewed Journals:
- Harris KM, Cruce WLR, Greenough WT, and Teyler TJ. (1980) A Golgi impregnation technique for thin brain slices maintained in vitro. J. Neurosci. Methods, 2:363 371.
- Harris KM. (1980) Relationships between dendrite and spine neck diameters in freeze fractured rat hippocampal formation. Biol. Bull. 159:470 471.
- Harris KM, Teyler TJ. (1983) Age differences in a circadian influence on hippocampal LTP. Brain Res., 261:69 71.
- Harris KM, Teyler TJ. (1983) Evidence for late development of inhibition in area CA1 of the rat hippocampus. Brain Res., 268:339 343.
- Harris KM, Teyler TJ. (1984) Developmental onset of long term potentiation in area CA1 of the rat hippocampus. J. Physiol., 346:27 48.
- Harris KM, Marshall PE, Landis DMD. (1985) Ultra¬structural study of cholecystokinin-immunoreactive cells and processes in area CA1 of the rat hippocampus. J. Comp. Neurol., 233:147 158.
- Harris KM, Landis DMD. (1986) Synaptic membrane structure in area CA1 of the rat hippocampus. Neuroscience 19:857 872.
- Harris KM, Stevens JK. (1988) Dendritic spines of rat cerebellar Purkinje cells: Serial electron microscopy with reference to their biophysical characteristics. Journal of Neuroscience, 8:4455-4469.
- Teyler TJ, Perkins AT IV, Harris KM. (1988) The development of long term potentiation in hippocampus and neocortex. Neuropsychologia, 27:31-39.
- Harris, KM and Stevens, JK (1989) Dendritic spines of CA1 pyramidal cells in the rat hippocampus: serial electron microscopy with reference to their biophysical properties. Journal of Neuroscience 9:2982-2997. (Cover Illustration)
- Jensen, FE and Harris, KM (1989) Preservation of neuronal ultrastructure in hippocampal slices using rapid microwave-enhanced fixation. J. Neurosci. Methods, 29:217-230.
- Harris KM, Jensen FE, and Tsao B. (1992) Three-dimensional structure of dendritic spines and synapses in rat hippocampus (CA1) at postnatal day 15 and young adult ages: Implications for the maturation of synaptic physiology and long term potentiation. Journal of Neuroscience, 12:2685-2705.
- Chicurel M and Harris KM. (1992) Three-dimensional analysis of the structure and composition of CA3 branched dendritic spines and their synaptic relationships with mossy fiber boutons in the rat hippocampus. J. Comp. Neurol. 325:169-182.
- Harris KM, Rosenberg PA. (1993) Localization of Synapses in Rat Cortical Cultures. Neuroscience 53:495-508.
- Lisman, J. and Harris, KM (1993) Quantal analysis and synaptic anatomy - integrating two views of hippocampal plasticity. Trends in Neuroscience, 16:141-147. (Cover Illustration)
- Sorra K and Harris KM (1993) Occurrence and three-dimensional structure of multiple synapses between individual radiatum axons and their target pyramidal cells in Hippocampal area CA1. Journal of Neuroscience, 13(9): 3736-3748.
- Jackson PS, Suppes T and Harris KM (1993) Rapid changes in the pattern and duration of long-term potentiation at postnatal days 11 and 15 in the rat hippocampus. J. Neurophysiology, 70(4):1412-1419.
- Carlbom I, Terzopoulos D, and Harris KM (1994) Computer-assisted registration, segmentation, and 3D reconstruction from images of neuronal tissue sections. IEEE Transactions on Medical Imaging, 13:351-362.
- Lisman, J. and Harris, KM (1994) Who's been nibbling on my PSD; is it LTD? J. Physiol. (Paris), 88:193-195.
- Harris, KM (1995) How Multiple Synapse Boutons Could Preserve Input Specificity During an Interneuronal Spread Of Long-term Potentiation. Trends in Neuroscience 18:365-369.
- Harris KM and Sultan P (1995) Variation in the Number, Location and Size of Synaptic Vesicles Provides an Anatomical Basis for the Nonuniform Probability of Release at Hippocampal CA1 Slices. J. Neuropharm. 34(11):1387-1395.
- Spacek J and Harris KM (1997) (Cover Illustration) Three-Dimensional Organization of Smooth Endoplasmic Reticulum in Hippocampal CA1 Dendrites and Dendritic Spines of the Immature and Mature Rat. J. Neurosci. 17: 190-203.
- Ouyang, Y, Schuman, EM, Harris, KM and Kennedy, MB (1997) Visualization of the distribution of autophosphorylated calcium/calmodulin-dependent protein kinase II after tetanic stimulation in the CA1 area of the hippocampus. J. Neuroscience 17:5416-5427.
- Finkbeiner S, Tavazoie S, Maloratsky A., Harris KM and Greenberg ME. (1997) CREB: A Major Mediator of Neuronal Neurotrophin Responses. Neuron, 19:1031-1047.
- Sorra KE, and Harris, KM (1998) Stability in synapse number and size at two hours after long-term potentiation in hippocampal area CA1. J. Neuroscience 18(2): 658-671.
- Spacek, J. and Harris KM. (1998) Three dimensional organization of cell adhesion junctions at synapses and dendritic spines in area CA1 of the Rat Hippocampus. J. Comp. Neurol. 393:58-68.
- Sorra, KE Fiala, JC and Harris, KM (1998) Critical assessment of the involvement of perforations, spinules, and spine branching in hippocampal synapse formation. J. Comp. Neurol. 398:225-240.
- Shepherd, GMG and Harris, KM (1998) Three-dimensional structure and composition of CA3?CA1 Axons in rat hippocampal slices: Implications for presynaptic connectivity and compartmentalization. J Neurosci. 18: 8300-8310.
- Fiala, JC, Feinberg, M. Popov, V. and Harris, KM (1998) Synaptogenesis via dendritic filopodia in developing hippocampal area CA1. J Neurosci. 18(21):8900-8911.
- Kirov, SA, Sorra, KE, Harris, KM (1999) Slices have more synapses than perfusion-fixed hippocampus from both young and mature rats. J Neurosci. 19(8):2876-2886.
- Ventura R, and Harris KM (1999) Three-Dimensional Relationships between Hippocampal Synapses and Astrocytes. J Neurosci. 19(16):6897-6906.
- Kirov SA and Harris KM (1999) Dendrites are more spiny on mature hippocampal neurons when synapses are inactivated. Nat Neurosci 2(10):878-883.
- Fiala JC, Harris KM (2001) Extending unbiased stereology of brain ultrastructure to three-dimensional volumes. J Amer Med Inform Assoc. 8(1):1-16.
- Fiala JC, Harris KM (2001) Cylindrical diameters method for calibrating section thickness in serial electron microscopy. J of Microscopy. 202(Pt 3):468-72.
- Xu-Friedman MA, Harris KM, Regehr WG. (2001) Three-Dimensional Comparison of Ultrastructural Characteristics at Depressing and Facilitating Synapses onto Cerebellar Purkinje Cells. J Neurosci. 21(17): 6666-72.
- Cooney J, Hurlburt J, Selig D, Harris KM and Fiala JC (2002) Endosomal compartments serve multiple hippocampal dendritic spines from a widespread rather than a local store of recycling membrane. J Neurosci. 22(6):2215-24.
- Fiala JC, Allwardt B, and Harris KM (2002) Dendritic spines do not split during hippocampal LTP or maturation. Nat Neurosci. 5(4): 297-8.
- Ostroff LE, Fiala JC, Allwardt B, Harris KM (2002) Polyribosomes redistribute from dendritic shafts into spines with enlarged synapses during LTP in developing rat hippocampal slices. Neuron. 35(3):535-545.
- Fiala JC, and Harris KM (2002) Computer-based alignment and reconstruction of serial sections. Microscopy and Analysis. 87:5-8.
- Fiala JC, Spacek J, Harris KM (2002) Dendritic spine pathology: cause or consequence of neurological disorders? Brain Res Rev. 39(1):29-54.
- Henze DA, McMahon, DBT, Harris KM, and Barriounuevo G (2002) Giant miniature EPSCs at the hippocampal mossy fiber to CA3 pyramidal cell synapse are monoquantal. J Neurophysiol. 87(1):15-29.
- Harris KM, Fiala JC, Ostroff L. (2003) Structural changes at dendritic spine synapses during long-term potentiation. Philos Trans R Soc Lond B Biol Sci. 358:745-8.
- Fiala JC, Kirov SA, Feinberg MD, Petrak LJ, George P, Goddard CA, and Harris KM (2003) Timing of Neuronal and Glial Ultrastructure Disruption During Brain Slice Preparation and Recovery In Vitro. J Comp Neurol 465:90-103.
- Spacek, J and Harris, KM (2004) Trans-endocytosis via Spinules in Adult Rat Hippocampus. J Neuroscience 24(17):4233-41; and featured in “This week in the journal.”
- Kirov, S.A., Harris K.M. (2004) Serial electron microscopy, confocal microscopy and two-photon microscopy as complementary tools for the study of synapses and dendritic spines in the central nervous system. Microscopy and Microanalysis 10 (Suppl. 2), 222-223.
- Kirov SA, Petrak LJ, Fiala JC, and Harris KM (2004) Dendritic Spines Disappear When Mature Hippocampal Slices Are Chilled But Proliferate Excessively Upon Re-warming. Neuroscience 127:69-80.
- Kirov SA, Goddard, CA and Harris KM (2004) Age-Dependence in the Homeostatic Up-regulation of Hippocampal Dendritic Spine Number During Blocked Synaptic Transmission, Neuropharmacology 47:640-648.
- Petrak LJ, Harris KM, Kirov SA (2005) Synaptogenesis on mature hippocampal dendrites occurs via filopodia and immature spines during blocked synaptic transmission. J. Comp. Neurol. 484:183-90.
- Seid, MA, Harris, KM and Traniello JFA (2005) Age-related changes in the number and structure of synapses in the lip region of the mushroom bodies in the ant Pheidole dentata. J. Comp. Neurol. 488:269-277.
- Sorra, KE, Mishra, A, Kirov SA and Harris, KM (2006) Dense core vesicles resemble active-zone transport vesicles and are diminished following synaptogenesis in mature hippocampal slices. Neuroscience 141:2097-2106.
- Park M, Salgado JM, Ostroff LE, Helton TD, Robinson CG, Harris KM and Ehlers ME (2006) Plasticity-induced growth of dendritic spines by exocytic trafficking from recycling endosomes. Neuron 52(5):817-30. Cover. Also, featured in Science Perspectives: Kopec and Malinow (2006) Matters of Size. Science 314:1554-55.
- Harris KM, Perry E, Bourne J, Feinberg M, Ostroff L, Hurlburt J (2006) Uniform serial sectioning for transmission electron microscopy. Journal of Neuroscience 26(47):12101-3. Cover.
- Witcher MR, Kirov SA and Harris KM (2007) Plasticity of perisynaptic astroglia during synaptogenesis in the mature rat hippocampus. Glia 55(1):13-23. Cover.
- Bourne JN, Sorra KE, Hurlburt J and Harris KM (2007) Polyribosomes are increased in spines of CA1 dendrites 2 h after the induction of LTP in mature rat hippocampal slices. Hippocampus 17(1):1-4.
- Bourne JN, Kirov SA, Sorra KE, Harris KM (2007) Warmer preparation of hippocampal slices prevents synapse proliferation that might obscure LTP-related structural plasticity, Neuropharmacology 52(1):55-9.
- Invited Reviews and Book Chapters:
- Harris KM, Stevens JK. (1988) Study of dendritic spines by serial electron microscopy and three-dimensional reconstructions. Neurol. and Neurobiol. 37:179-199.
- Harris, KM, Jensen FE, and Tsao B. (1989) Ultrastructure and Plasticity of spine synapses in area CA1 of the rat hippocampus: Extending our vision with serial electron microscopy and quantitative three dimensional analyses. Neurol. and Neurobiol. 52:33-52.
- Carlbom I, Terzopoulos D. and Harris KM. (1991) Reconstructing and visualizing models of neuronal dendrites. In: Patrikalakis, NM (Ed.) Scientific Visualization of Physical Phenomena. Springer-Verlag, New York.
- Harris, KM and Kater, S. (1994) Dendritic spines: Cellular specializations that impart stability and flexibility to synaptic function. Ann. Rev. Neurosci. 17:341-371.
- Harris, KM (1994) Serial Electron Microscopy as an Alternative or Complement to Confocal Microscopy. In: Stevens, J.K., Mills, L.R., and Trogadis, J.E. (Eds.) Three Dimensional Confocal Microscopy: Volume Investigation of Biological Specimens, Academic Press: New York, pp 421-445, and color section 4.
- Harris, KM (1998) Dendritic Spines. Encyclopedia of Life Sciences. Macmillan Reference Ltd, London.
- Harris, KM (1999) Dendritic Spines. Encyclopedia of Neuroscience, 2nd Edition. G. Adelman and B. Smith Editors.
- Harris, KM (1999) Structure, Development, and Plasticity of Dendritic Spines. Current Opinion in Neurobiology 9:343-348.
- Fiala, JC and Harris, KM (1999) Structure of Dendrites and Spines. In: Stuart et al., (Eds) Dendrites. Oxford University Press, Oxford UK.
- Harris, KM (1999) Calcium from internal stores modifies dendritic spine shape. Proc. Natl. Acad. Sci. Vol. 96: 12213-12215.
- Eichenbaum, HE and Harris, KM (2000) Toying with memory in the hippocampus. News and Views, Nature Neuroscience 3:205-206.
- Sorra KE and Harris, KM (2000) Overview on the Structure, Composition, Function, Development, and Plasticity of Hippocampal Dendritic Spines. In: Eichenbaum HB (Ed.) with Harris KM and Sorra KE (Special Issue Eds.) Dendritic Spines of the Hippocampus. Hippocampus 10: 501-511. Cover Illustration.
- Feinberg, MD, Szumowski KM, Harris KM (2001) Microwave fixation of rat hippocampal slices. In: RT Giberson, RS DeMaree Jr. (eds.) Microwave Techniques and Protocols. Humana Press: Totowa, New Jersey. pp. 75-88.
- Harris, KM. (2003) Dendritic Spines. Encyclopedia of Neuroscience, 3rd Edition. Ed. G. Adelman and B. Smith. Amsterdam; New York: Elsevier.
- Bourne, JN and Harris KM. (2006) Synaptic Structure: Postsynaptic Organization: Ultrastructural analysis of spine plasticity. Encyclopedia of Neuroscience, 4th Edition. Ed. G. Adelman and B. Smith. Amsterdam; New York: Elsevier. In press.
- Bourne JN and Harris KM (2006) Dendritic Spines. Encyclopedia of Life Sciences. 2nd Macmillan Reference Ltd, London.
- Fiala, JC, Spacek, J and Harris, KM (2007) Dendrite Structure. In: Stuart et al., (Eds) Dendrites. 2nd Edition, Oxford University Press, Oxford UK (In Press).
- Database entitled SynapseWeb funded by NIH/NIBIB/NIMH EB002170:
- Abstracts from work in progress (note only includes abstracts from unpublished work):
- B. Shi and K. M. Harris (2004) Web-enabled database of synapse structure and function. Society for Neuroscience Abstract #1032.4
- Ostroff and Harris (2004) Dynamic dendrites: More and bigger spines and synapses and proliferation of polyribosomes during early LTP. Society for Neuroscience Abstract # 637.8 .
- Shi B, Liu F, Harris KM (2005) Editor for Re-sectioning and Analyzing Reconstructed Objects while Viewing them in 3D. Society for Neuroscience Abstract #570.5.
- Bourne JN, Harris KM (2005) Spinules and LTP at Mature CA1-CA3 Synapses. Society for Neuroscience Abstracts #501.20.
- Risher WC, Ostroff LE, Harris, KM (2006) Size-dependent plasticity in the relationship between synapses and astroglia during LTP. Glia in Health and Disease, Cold Spring Harbor Laboratory, Cold Spring Harbor, NY.
- Risher WC, Ostroff LE, Harris, KM (2006) What dendritic filopodia induced by LTP encounter along their path through the neuropil of PN15 rat hippocampus. Society for Neuroscience Abstracts, 135.4.
- Vazdarjanova AI, Bunting K, Parkerson J, Lummer T, Journey C, Harris KM (2006) Recall of spatial remote memory induces Arc "a plasticity-related immediate-early gene" in hippocampus but not perirhinal or piriform cortex. Society for Neuroscience Abstracts, 371.1.
- Zhang W, Bartol TM, Harris KM, and Sejnowski TJ (2006) Monte Carlo model of presynaptic calcium dynamics of a hippocampal neuron. Society for Neuroscience Abstracts, 338.12.